Cell Transfection refers to the technology of transferring exogenous molecules such as DNA, RNA, proteins, etc. into eukaryotic cells, and has become a routine tool for studying and controlling the function of eukaryotic cells. It is widely used in biological experiments such as studying gene function, regulating gene expression, mutation analysis and protein production.
The ideal cell transfection method should have the advantages of high transfection efficiency and low cytotoxicity. Cell transfection is currently performed primarily through physical, chemical, and biological mediation methods. Physical methods include electroporation, microinjection, and gene guns; many chemically mediated methods, such as classical calcium phosphate coprecipitation, lipofection, and a variety of cationic material-mediated techniques; bio-mediated methods It mainly includes protoplast transfection and virus-mediated transfection techniques.