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Hotline: 400-686-0075

To cope with the time-consuming procedures of apoptosis detection, RiboBio has introduced the riboAPO, a one-step TUNEL apoptosis detection kit. using TUNEL method, which is exposed to TdT enzyme catalysis and exposure of apoptotic cell break genomic DNA fragments. The 3 ́-OH end of the catalyzed incorporation of fluorescein-dUTP allows for the detection of apoptosis in a single step without the need for additional fluorescent dyes and overnight manipulation, especially for the detection of advanced apoptosis or DNA fragmentation. The experiment can be completed in 1-3 hours.

Product advantage

1. Recommended for a wide range of samples: Sutitable for the detection of apoptotic cells in adherent cells, suspension cells and tissue sections
2. High sensitivity: The single-cell apoptosis before morphological changes can be detected
3. Easy to operate: One step reaction, no other color development steps or overnight operation needed
4. Rapid detection: A complete apoptotic cell detection only takes 1-3 hours
5. High signal to noise ratio: background staining Low, positive staining bright
6. Compatible with a variety of detection methods: Can be detected by fluorescence microscopy, flow cytometry, high-content and ultra-high resolution microscope

Detection Principle

Schematic Diagram of riboAPO™ One-Step TUNEL Apoptosis Kit(Green)

 

Schematic Diagram of riboAPO™ One-Step TUNEL Apoptosis Kit(Red)

Technical Support

1. Detection Result of One-Step TUNEL Apoptosis Kit (Green)


The one-step TUNEL kit (Green) is applied to detect staurosporine (STS)-induced apoptosis in A549 cells (experiment completed on IN Cell Analyzer 6500HS)


One-step TUNEL kit for detecting apoptosis of DNase I-treated A549 cells by fluorescence microscopy

2. Detection Result of One-Step TUNEL Apoptosis Kit (Red)

1) Apoptosis detection of adherent cells

“Adherent cell detection result with riboAPO™ One-Step TUNEL Apoptosis Kit (Red)
A. TUNEL staining positive cells were detected in A549 cells, regardless of the involvement of DNase I
B. TUNEL staining positive cells were detected in MHCC97H cells, regardless of the involvement of DNase I”

2) Apoptosis detection of paraffin-embedded tissue sections

Apoptosis detection result of paraffin-embedded tissue sections with riboAPO™ One-Step TUNEL Apoptosis Kit (Red)
Normal, normal mouse liver tissue;
Injury, cell apoptosis in liver ischemia-reperfusion injury in mice.

3) Apoptosis detection of suspension cells

Suspension cell detection result with riboAPO™ One-Step TUNEL Apoptosis Kit (Red)
A. TUNEL staining positive cells were detected in untreated THP-1 cells;
B. TUNEL staining positive cells were detected in THP-1 cells treated with DNase I for 15min

Ordering Information

SKU ProductList PriceQuantityActions
C11026-1 riboAPO One-Step TUNEL Apoptosis Kit (Red )(20T )$287.88
C11026-2 riboAPO One-Step TUNEL Apoptosis Kit (Red )(100T )$1,004.26
C11012-3 riboAPO One-Step TUNEL Apoptosis Kit (Green )(500T )$3,693.52
C11026-3 riboAPO One-Step TUNEL Apoptosis Kit (Red )(500T )$3,693.52
C11012-1 riboAPO One-Step TUNEL Apoptosis Kit (Green )(20T )$287.88
C11012-2 riboAPO One-Step TUNEL Apoptosis Kit (Green )(100T )$1,004.26
This product is for low temperature transportation and needs to be charged for low temperature freight. For details, please contact customer service online.

 

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Guangzhou RiboBio Co., Ltd.
Address:13-14/F, Innovation Building C3, 182 Kexue Avenue, Science Park, Guangzhou 510663, China
Service hotline:400-686-0075

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