To cope with the time-consuming procedures of apoptosis detection, RiboBio has introduced the riboAPO, a one-step TUNEL apoptosis detection kit. using TUNEL method, which is exposed to TdT enzyme catalysis and exposure of apoptotic cell break genomic DNA fragments. The 3 ́-OH end of the catalyzed incorporation of fluorescein-dUTP allows for the detection of apoptosis in a single step without the need for additional fluorescent dyes and overnight manipulation, especially for the detection of advanced apoptosis or DNA fragmentation. The experiment can be completed in 1-3 hours.
Product Advantage
1. Recommended for a wide range of samples: Sutitable for the detection of apoptotic cells in adherent cells, suspension cells and tissue sections
2. High sensitivity: The single-cell apoptosis before morphological changes can be detected
3. Easy to operate: One step reaction, no other color development steps or overnight operation needed
4. Rapid detection: A complete apoptotic cell detection only takes 1-3 hours
5. High signal to noise ratio: background staining Low, positive staining bright
6. Compatible with a variety of detection methods: Can be detected by fluorescence microscopy, flow cytometry, high-content and ultra-high resolution microscope
Detection Principle
Schematic Diagram of riboAPO™ One-Step TUNEL Apoptosis Kit（Green）
Schematic Diagram of riboAPO™ One-Step TUNEL Apoptosis Kit（Red）
Technical Support
1. Detection Result of One-Step TUNEL Apoptosis Kit (Green)
The one-step TUNEL kit (Green) is applied to detect staurosporine (STS)-induced apoptosis in A549 cells (experiment completed on IN Cell Analyzer 6500HS)
One-step TUNEL kit for detecting apoptosis of DNase I-treated A549 cells by fluorescence microscopy
2. Detection Result of One-Step TUNEL Apoptosis Kit (Red)
1) Apoptosis detection of adherent cells
“Adherent cell detection result with riboAPO™ One-Step TUNEL Apoptosis Kit (Red)
A. TUNEL staining positive cells were detected in A549 cells, regardless of the involvement of DNase I
B. TUNEL staining positive cells were detected in MHCC97H cells, regardless of the involvement of DNase I”
2) Apoptosis detection of paraffin-embedded tissue sections
Apoptosis detection result of paraffin-embedded tissue sections with riboAPO™ One-Step TUNEL Apoptosis Kit (Red)
Normal, normal mouse liver tissue;
Injury, cell apoptosis in liver ischemia-reperfusion injury in mice.
3) Apoptosis detection of suspension cells
Suspension cell detection result with riboAPO™ One-Step TUNEL Apoptosis Kit (Red)
A. TUNEL staining positive cells were detected in untreated THP-1 cells;
B. TUNEL staining positive cells were detected in THP-1 cells treated with DNase I for 15min
Ordering Information
| SKU | Product | List Price | Quantity | Actions |
|---|---|---|---|---|
| C11026-1 | riboAPO One-Step TUNEL Apoptosis Kit (Red )(20T ) | $287.88 | ||
| C11026-2 | riboAPO One-Step TUNEL Apoptosis Kit (Red )(100T ) | $1,004.26 | ||
| C11012-3 | riboAPO One-Step TUNEL Apoptosis Kit (Green )(500T ) | $3,693.52 | ||
| C11026-3 | riboAPO One-Step TUNEL Apoptosis Kit (Red )(500T ) | $3,693.52 | ||
| C11012-1 | riboAPO One-Step TUNEL Apoptosis Kit (Green )(20T ) | $287.88 | ||
| C11012-2 | riboAPO One-Step TUNEL Apoptosis Kit (Green )(100T ) | $1,004.26 |
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