LNA (Locked nucleic acid) is one of the modified nucleic acid analogues that have good identification ability and affinity to DNA and RNA, which locks the ribose ring into an ideal rigid conformation of “Watson-Crick”. When LNA hybridizes with complementary DNA or RNA chain, it will show the extremely high thermal stability. When one LNA monomer is inserted, duplex Tm will increase 2-8°C, which is especially suitable for detection on DNA or RNA targets with short length or high similarities.
LNA base can be inserted into any position for any sequence including probe, RNA sequence, normal DNA sequence and various modified sequences. RiboBio can provide oligonucleotides chemical synthesis and modification services based on the LNA modification provided by clients.
Product Features
The 2′-O and 4′-C atoms of the LNA ribosome will form into a methylene bridge via various shrinking effects, connect into a ring, essentially locking the structure into a rigid conformation of “Watson-Crick”. When DNA or RNA Oligos are inserted, LNA can quickly match the complementary nucleotide chain and improve the stability for the obtained duplex.
Generally, LNA Oligos are made up with LNA and DNA or RNA mixture. Therefore, the LNA content in Oligos can be altered to optimize sensitivity and specificity. LNA Oligos is able to improve the sensitivity and specificity for hybridization-based technologies (including PCR, microarray and in situ hybridization).
Extensive Applications
LNA has extensive applications, which can perform specific and sensitive detection on non-coding RNA and other small RNA molecules. LNA Oligos is able to differentiate highly similar sequence, which can also be used in longer RNA sequences such as mRNA. In addition, LNA is also applicable to detect on nucleic acid with low expression and chromosome DNA.
Typical Application Areas
DNA Analysis ：
1. Real-time PCR
2. Allele-specific PCR Detection on SNP
3. Applications Based on Magnetic Beads
4. Chromosome FISH
5. Comparative Genome Hybridization
6. Proteomics of Isolated Chromatin Fragments (PICh)
7. Antigen Inhibition
8. Mutagenesis
mRNA Analysis ：
1. Real-time PCR
2. Microarray Analysis
3. In Situ Hybridization
4. Northern Blot Analysis
5. Applications Based on Magnetic Beads
6. Fluorescence-activated Cell Sorting (FACS)
7. RNA Function Inhibitory Research
8. RNA Modifications (frame shift, exon skipping)
9. DNAzymes
ncRNA Analysis ：
1. Real-time PCR
2. Microarray Analysis
3. In Situ Hybridization
4. Northern Blot Analysis
5. Fluorescence-activated Cell Sorting (FACS)
6. RNA Function Inhibitory Research
7. RNA Modifications (frame shift, exon skipping)
miRNA Analysis ：
1. Real-time PCR
2. Microarray Analysis
3. In Situ Hybridization
4. Northern Blot Analysis
5. Applications Based on Magnetic Beads
6. RNA Function Inhibitory Research
