Based on various samples and detection requirements, RiboBio can provide high-quality qRT-PCR services by utilizing Bulge-Loop™ miRNA qRT-PCR and miDETECT A Track™miRNA qRT-PCR to obtain the reliable results and good repeatability.
Process
a）RNA Sample Preparation
b）RNA Quality Control
c）cDNA Synthesis and Reversely Transcription
d）Real-time PCR
e）Result Analysis and Provision of Experimental Report
Method and Instrument
Use Bio-Rad CFX96 to perform SYBR Green Fluorescence quantitative detection.
Services
a) Relative Quantification: Take U6 snRNA or 5S rRNA as internal control to detect the miRNA expression in the samples;
b) Absolute Quantification: Take chemically synthesized mature miRAN as standard, prepare 5 gradients or above, dilute and prepare quantitative standard curve. Perform absolute quantification on uninspected miRNA through standard curve.
Case Studies
Amplification Curve Detected by Bulge-Loop™ miRNA qRT-PCR
Melting Curve Detected by Bulge-Loop™ miRNA qRT-PCR
