Biomarker refers to a biochemical indicator to label the changes or possible changes in the system, organ, tissue, cell and subcellular structure or function, and can serve a very wide range of purposes. RiboBio provides cell imaging-based markers, co-localization and biochemical testing services.
In multi-labeled fluorescence sample images, co-localization refers to a superposition of emission signals, and is often caused by the close distribution of two or more fluorophores in the microstructure. The co-localization of tissue sections, single cells, or subcellular organs seen in a microscope means that different molecules are attached to the same receptor.
RiboBio perform markers, co-localization and biochemical analysis and screening:
■Tumor markers
■Hemoglobin
■ Dissolved oxygen level (ROS)
■Calcium ion
■Oxygen molecules and reactive oxygen species
■DNA damage (and repair)
■Fluorescence in situ hybridization FISH
Examples
Fluorescence in situ hybridization (FISH) analysis:
The localization of lncRNA MIR31HG in hASCs was specifically detected with RiboBio’s lncRNA FISH Probe Mix, and the changes before and after the stimulation of TNF-α and IL-17 were compared. The U6 detected by h-U6 FISH Probe Mix was located in the nucleus, while the 18S detected by the h-18S FISH Probe Mix was in the cytoplasm. Jin C, et al. Stem Cells, 2016.