Exosomes widely exist in body fluids such as supernatant and peripheral blood, urine, ascites, which can come from various types of cells. Exosomes content contains protein, lipid, nucleic acid, etc. Due to the lipid package and protection, its stability and biological function can still be remained for exosomes content. Exosomes RNA, especially non-coding RNA, will have remarkably differential expression in different disease models, which will be expected to become the important molecule marker for liquid biopsy and disclose disease mechanism. Using high-throughput transcription sequencing can simultaneously detect its RNA molecules such as lncRNA, mRNA, which will help the study on identification and regulatory function of exosomes mRNA, lncRNA, etc.
Advantages
1. Patented exosome isolation technology with specifically mirco RNA library building method and provides lncRNA overall sequencing services
2. 3 exosomes or vesicles databases with more complete annotations to provide evidence for further studies
3. Various calculation methods for differential expressions. Select the suitable method based on specific project and demonstrate more perfect data
4. Brand-new alignment software with fast analysis speed and more accurate results
5. Provide a more comprehensive clinical data analysis for oncology study
Overall Technical Route for Exosomes Research
High-throughput Sequencing and Analysis Process
Bioinformatics Analytical Items
FAQ
1. Besides lncRNA sequencing and analysis for exosomes, does RiboBio also provide exosomes isolation services? Are there any requirements for samples?
By using RiboTM Exosome Isolation Reagent developed by RiboBio, we provide exosomes isolation services for human, mice, rats, supernatant, serum, plasma and other body fluids. After large amount of experimental verifications, RiboTM Exosome Isolation Reagent can efficiently extract exosomes with complete structures and functions and the products can be used for downstream experiments including NGS, cell co-cultivation and in vivo injection.
2. Does cell culture state affect the exosomes sequencing result?
Yes. Different culture conditions will cause various amount of exosomes by cell secretion. During the cell apoptosis, large amount of apoptosis body and cell debris will be released. Apoptosis body is also vesicular structure that is difficult to be eliminated during exosomes isolation, which will affect the sequencing data.
Are there any requirements for samples?
