Small RNA is a classification of RNA molecules including miRNA, piRNA and snoRNA. Small RNA is involved in gene expression and regulation, RNA processing and slicing, protein translation, genetic “invasion” inhibition, gametogenesis and other important biological processes, thus being an indispensable regulatory factor in life activities. MicroRNAs (miRNAs) are the major part of small RNA and can be used to regulate post-transcriptional gene regulation by targeting mRNAs.
Advantages
1. Patented exosome extraction technology combined with unique microRNA library construction methods to provide miRNA sequencing services
2. 3 exosomes or vesicle database applied, the annotation information is more perfect, able to validate further research
3. Clinical data analysis of tumor research making the results more comprehensive
4. 4 latest databases to predict target genes
Common Functions of miRNAs in Exosomes
Small RNA is a classification of RNA molecules including miRNA, piRNA, tsRNA and snoRNA. Small RNA is involved in gene expression and regulation, RNA processing and slicing, protein translation, genetic “invasion” inhibition, gametogenesis and other important biological processes, thus being an indispensable regulatory factor in life activities. MicroRNAs (miRNAs) are the major part of small RNA and have been found to be closely related to cancer, cardiovascular disease and immune system diseases. For example, miRNAs can be transported by exosomes to other cellular tissues to create a suitable microenvironment for cancer cell metastasis, thereby promoting cancer cell metastasis. Also, exosome miRNAs can be used as biomarkers for diseases, and are widely used in disease diagnosis, personalized treatment and prognosis. High-throughput sequencing technology can obtain the sequence information of all small RNAs from the sample in a single sequencing process, so as to analyze the expression levels of small RNAs with different disease states, sample sources and processing conditions, and to accurately compare the slight differences. In this way, it offers biological significance for the future study of small RNAs.
Bioinformatics Analysis
RiboBio can provide multiple bioinformatics analysis services including bascis and advanced analysis services, welcome to contact order@ribobio.com to know more about it.
FAQs
1. Is it possible to conduct small RNA sequencing with species that don't involve a reference genome, or miRNA sequences whose miRBase does not include a reference species?
Species without a reference genome can be used as a reference-free transcriptome splicing transcript, with the transcript being a reference sequence for small RNA sequencing analysis, or a genome of related species can also be used as a reference. miRBase does not include miRNA sequences for species to be studied, and the miRNA sequences of closely related species can be annotated.
2. What are the precautions for exosome sample collection?
Samples should not be added with any RNA protectant (eg TRIzol, RNAlater) prior to the separation of exosomes.
After the blood sample is collected, the whole blood should be gently dripped into a clean EP tube or a centrifuge tube;
Centrifugation should be performed when the blood clots revealed after being stored at 4°C for 3-4 hours.
Plasma samples should not be anticoagulated with heparin, and should be mixed with the whole blood using a blood collection needle and an EDTA anticoagulation tube;
The supernatant should be removed through centrifugation after being stored at 4°C for 3-4 hours.
During the cell culture process, serum removed with exosomes should be applied for cell supernatant samples to avoid mycoplasma contamination. For more detailed sample collection procedures, please refer to the “Sample Collection Process”.
