Methylated RNA Immunoprecipitation Sequencing (MeRIP-Seq) combines RNA-protein immunoprecipitation and high-throughput sequencing to achieve RNA methylation analysis at the full transcriptome level. Methylation modification accounts for more than 60% of all RNA modifications, and N6-methyladenosine (m6A), as the most common post-transcriptional modification, mediates more than 80% of RNA methylation. Therefore, the analysis of RNA methylation patterns based on MeRIP-Seq accelerates the study of biological functions and mechanisms of cancer development and metastasis, embryo development, lipid metabolism, circular RNA translation and DNA damage repair. RiboBio uses monoclonal antibodies to capture m6A-modified RNA fragments and combines high-throughput sequencing to achieve m6A peak detection and motif analysis in the transcriptomics range.
Advantages
● Platform integrity: One-stop service for MeRIP experiment, database analysis, and MeRIP qPCR verification
● High success rate: More than 1000 sample practices, supporting the ng-level RNA library construction
● Multiple selection: Various library construction methods and fragment sizes optional
● Repeatible: Rich project experience to ensure repeatability and comparative analysis between multiple samples
References of Examples
[1]Wang H, Hu X, Huang M, et al. Mettl3-mediated mRNA m 6 A methylation promotes dendritic cell activation[J]. Nature communications, 2019, 10(1): 1898.
[2]Li T, Hu P S, Zuo Z, et al. METTL3 facilitates tumor progression via an m 6 A-IGF2BP2-dependent mechanism in colorectal carcinoma[J]. Molecular cancer, 2019, 18(1): 112.
Bioinformatics Analysis
RiboBio can provide multiple bioinformatics analysis services including bascis and advanced analysis services, welcome to contact order@ribobio.com to know more about it.
FAQs
1. Is there species restriction for MeRIP-seq?
For MeRIP-seq, species with reference genomes which are spliced to chromosome levels, and complete gtf annotation files are operationable. There are differences in the software and parameters involved in the peak detection of eukaryotes, prokaryotes or virals, and prokaryotic or viral projects are recommended for priority evaluation.
2. Why does the MeRIP project require both Input and IP sample detection?
In MeRIP projects, Input and IP are paired samples. The IP sample specifically enriches methylated RNA fragments with m6A antibody, while the Input was only fragmented RNA that which was used as a control to reduce the background noise, and was carried out in parallel with database construction and sequencing. Peak detection analysis requires integrating the data of two samples and using Input data to exclude peaks with high background expression or non-specific binding to improve the accuracy of calling peak.
