Metagenomics sequencing is a high-throughput sequencing of microbial community genomes in specific environmental samples to analyze the genetic composition and function of microbial populations, interpret the diversity and abundance of microbial populations, and explore the relationship between microbes and the environment, between microorganisms and hosts, so as to discover and research new, specific functional genes. The metagenomics sequencing research is free from microbial isolation and culture and expands the utilization space of microbial resources, providing an effective tool for microbial research.
Advantages
● High detection accuracy, can detect single base differences; more comprehensive information, can be found for high and low abundance species genes
● High throughput, fast cycle, single sequencing for all species in the sample
● Provide comprehensive antibiotic factor analysis and provide customized analysis according to customer needs
Bioinformatics Analysis
RiboBio can provide multiple bioinformatics analysis services including bascis and advanced analysis services, welcome to contact order@ribobio.com to know more about it.
FAQs
1. What are the requirements for sample preparation for metagenomic sequencing?
For metagenomics sequencing, more than 3g of DNA samples with a concentration of ≥30ng/μl are required, and it should be free from protein, RNA or visible contamination. A gel electropherogram is also needed, and its main display band shoudl be clearly distinguishable.
2. What are the advantages of metagenomics sequencing compared to single individual studies?
Microorganisms are usually symbiotic in a small habitat in a community, with their characteristics generally based on the entire community environment and the interaction between individuals. Therefore, the metagenomics research is more efficient in studying its characteristics than single individual research. Metagenomics research doesn’t require the isolation of individual bacterias, and it is suitable for studying microorganisms that cannot be isolated and cultured in labs.
3. What is the difference between 16S rDNA sequencing and metagenomic de novo sequencing?
16S rDNA sequencing is a PCR amplification of specific hypervariable regions of bacterial ribosomal small subunits, reflecting the evolutionary relationship and community diversity of species. The metagenomic de novo sequencing determines the genome of all microorganisms in the whole environment, which not only reflects the evolutionary relationship of species and community diversity, but also analyzes species composition and functional annotations. It is more comprehensive than the analyzable levels of 16S rDNA sequencing, and the cost is also higher.
