Small RNA is a classification of RNA molecules such as miRNA, piRNA, tsRNA and snoRNA. Small RNA is involved in gene expression and regulation, RNA processing and slicing, protein translation, genetic “invasion” inhibition, gametogenesis and other important biological processes, thus being an indispensable regulatory factor in life activities. MicroRNAs (miRNAs) are the major part of small RNAs that help with post-transcriptional gene regulation by recognizing target mRNAs, and have been found to be closely related to cancer, cardiovascular disease and immune system diseases. Thanks to the application of small RNA sequencing technology in recent years, more and more miRNAs have been discovered and functionally identified, and are also widely used in the fields of disease diagnosis, personalized treatment and prognosis.
RiboBio offers a variety of Small RNA sequencing services depending on the extraction method, difficulty and type of database construction:
● Small RNA Sequencing of Tissue and Cell
● Small RNA sequencing of Exosomes
Advantages
- Single base differences can be detected to distinguish miRNAs of the same family or with high sequence similarity
- Self-developed data analysis methods and algorithms to identify known miRNAs and predict new miRNAs
- Multiple small RNA analysis of extended non-miRNAs help maximize data utilization
Bioinformatics Analysis
RiboBio can provide multiple bioinformatics analysis services including bascis and advanced analysis services, welcome to contact order@ribobio.com to know more about it.
FAQs
1. Is it possible to conduct small RNA sequencing with species that don't involve a reference genome, or miRNA sequences whose miRBase does not include a reference species?
Species without a reference genome can be used as a reference-free transcriptome splicing transcript, with the transcript being a reference sequence for small RNA sequencing analysis, or a genome of related species can also be used as a reference. miRBase does not include miRNA sequences for species to be studied, and the miRNA sequences of closely related species can be annotated.
2. Can RiboBio provide verification services for the results of Small RNA sequencing analysis?
RiboBio provides one-stop miRNA services from validation to functional research, including qPCR detection, target gene validation, and a variety of miRNA mimic, inhibitor, agomir, and antagomir products.
3. Apart from miRBase, is there any other comparison method for the miRNA sequencing data?
miRBase is the most commonly used miRNA database. It provides a comprehensive database of miRNA sequence data, annotations, and predicted gene targets, and is one of the most authoritative data sources for miRNA research. In addition to the miRBase database, RiboBio also uses more than 30 other miRNA databases on animals, plants, microbes and human for miRNA validation, structural prediction and target gene prediction.
4. Does miRNA high-throughput sequencing require sequence information in advance?
No. High-throughput sequencing of miRNAs can be applied to both reference and non-reference genomic species. For reference genomic species, customers are recommended to provide reference genomes for known miRNA expression and differential expression analysis and predictive analysis of candidate new miRNAs; for non-reference genomic species, miRNAs can be obtained based on homologous sequence alignments, but the new miRNAs cannot be predicted.
