Whole genome re-sequencing is the sequencing of genomes between different individuals of species with known reference genome sequences, and the individual or population differences will be analyzed. Through genome-wide re-sequencing, researchers can find a large number of variation information such as single nucleotide variant (SNV), copy number variation (CNV), insertion loss (InDel, Insertion/Deletion) and structural variation (SV), the application range includes clinical medicine research, population genetics research, inter-group association analysis, evolution analysis and others.
With the dramatic reducing cost and the increasing efficiency of sequencing, genome-wide resequencing has become one of the fastest and most effective methods for studying human diseases and animal and plant molecular breeding. It can help detect disease-related mutation sites, structural variability and other information at the genome-wide level, and find solutions for these diseases and develop effective therapeutic drugs.
Advantages
● Able to customize personalized analysis solutions according to different research purposes and directions
● Strict data quality control to ensure limited data deviation, high uniformity, and genomic information of true response samples
● Advanced analysis of multiple disease research
Project Procedures
1. Obtain genomic DNA
2. Library construction
3. On-line sequencing
4. Data quality control & comparison
5. Data analysis
Bioinformatics Analysis Projects
Know more about bioinformatics, please contact order@ribobio.com.
FAQs
1. What is the requirement of genomic re-sequencing depth?
Sequencing Depth refers to the ratio of the total number of bases (bp) and the genome size (Genome) obtained by sequencing. It is one of the indicators for evaluating the amount of sequencing. As the sequencing depth and genome coverage are positively correlated, the error rate or false positive result from sequencing will decrease as the sequencing depth increases.
2. How to verify the results of re-sequencing?
SNP, SV, CNV, InDel and other genetic variations can be identified through genome-wide resequencing, and different variation requires different verification methods.
1) SNPs can be amplified by PCR and sequenced by the SNP site; or verified by SNP typing.
2) CNVs (Copy Number Variants) can be amplified with Real-time PCR, and estimate the variation scope of different individuals according to CT values.
3) Small SVs can be identified by PCR amplification and sequencing, while large SVs need to be discovered by submicroscopic methods, such as FISH.
4) Small fragments of InDels can be amplified by PCR and verified by Sanger sequencing.
