Nucleic Acid In Vitro Transcription takes DNA as template in the in vitro system without cells, simulates the in vivo transcription process to generate RNA under the action of series of transcription factors in the transcription system. Nucleic Acid In Vitro Transcription reaction can be used to control the transcription gene, transcription process and post-transcription RNA usage. Ribobio provides nucleic acid in vitro transcription kits including Ribo™ RNAmax-T7 In Vitro Transcription kit/ Ribo™ RNAmax-T7 Biotin-labeled In Vitro Transcription kit, which can synthesize complementary RNA from DNA template (antisense strand). It can easily, quickly and stably obtain large amount of RNA molecules, such as mRNA, lncRNA, shRNA. Synthesized RNA can be used for RNA functional study.
RNA in Vitro Transcription is mainly used to synthesize the labeled or unlabeled RNA molecules, which are applicable to microinjection, in vitro translation and transfection experiments.
Synthesize virus RNA fragment for virus infectivity study
Synthesize siRNA for interference
Synthesize ribozyme to specifically cleavage target RNA sequence
Synthesize RNA probes for hybrid experiment
Synthesize mRNA for in vitro translation and transfection experiment
Synthesize non-coding RNA (eg. lncRNA) for RNA functional study
Synthesize biotin-labeled RNA molecules for RNA functional verification experiment (eg. RNA pull down)