Single Gene siRNA Set contains designed 3 pairs of siRNAs for each target gene (human, mouse, rat only), ensuring that at least 1 pair of siRNA mRNA levels were silenced by more than 70% under standard conditions of use. The set also comes with a negative control, a positive control, a positive control primer (human, mouse, rat only).
Economic Single Gene siRNA Set
The set has designed 3 pairs of siRNAs (5 nmol each) for each target gene (human, mouse, rat only), ensuring that at least 1 pair of siRNA mRNA levels were silenced by more than 70% under standard conditions of use. The pack also included a negative control of 5 nmol and a transfection control of 1 nmol; or a negative control of 5 nmol, a positive control of 5 nmol, and a positive control primer of 1 nmol (human, mouse, rat only).
Special Single Gene siRNA Set
The set has designed 3 pairs of siRNAs (5 nmol each) for each target gene (human, mouse, rat only), ensuring that at least 1 pair of siRNA mRNA levels were silenced by more than 70% under standard conditions of use. The pack also included a negative control 5nmol, a positive control 5nmol, a positive control primer 1nmol, a transfection control 1nmol, and a riboFECT CP transfection reagent 0.5ml.
1. Designed with exclusive patented technology, siRNA can achieve a silencing effect of 70% or more;
2. Design synthetic siRNA for more regions of the same mRNA to improve the success rate of silencing target genes;
3. More cost-effective, economic and affordable
4. After-sales service is complete, if the silencing effect is not good, re-synthsis 2 pair of siRNA for free by comprehensive analysis.
Silencing effect after-sales commitment:
If the 3 pairs of siRNAs are not able to achieve 70% silencing effect by qPCR identification, RiboBio will analyze the experimental data (transfection efficiency picture and qPCR result) provided from users. If the mRNA silencing efficiency does not reach 70%, then another two pairs of siRNAs against the target gene are redesigned and synthesized free of charge.
Which siRNA is right for you？
You only need to provide Gene Symbol and Gene ID. RiboBio will design and synthesize 3 pairs of siRNAs for this gene, and also provide appropriate negative control, positive control and positive control or PCR primers for customers to screen for effective siRNA.
Figure 1. siRNA pack for single-gene usually design 3 pairs of siRNAs for target genes.
A549 cell lines were transfected with 3 pairs of siRNA and Ncontrol of human EGFR gene at a final concentration of 50 nM, and siRNA silencing effect was detected by qPCR method 48 hours later. The results showed that the silencing effect of siRNA mRNA level reached 70%, and the expression of EGFR protein was significantly reduced by WB method after 72 hours.
Figure 2 qPCR detection of EGFR gene siRNA silencing effect
Figure 3 Western blot analysis of EGFR single gene siRNA kit silencing effect
Tips: The default positive control type for human 1999A and 2500A single-gene Pack siRNA is h-GAPDH (5nmol),
If you need other types of controls or design single-gene siRNA pack for other species, please go to design a custom product.
Custom Oligo Synthesis Click for custom Oligos
Product Design Click for designed products if you know gene name or ID.
Product brochure download
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