对lncRNA/基因进行过表达,进行功能获得性实验(gain-of-function)是研究基因功能的重要手段,与以RNAi为代表的功能缺失性实验(loss-of-function)相互补充以说明lncRNA/基因在相关细胞功能中的作用。锐博生物pEXP-RB-Mam系列过表达载体,在多克隆位点上游有CMV强启动子,可高效的表达插入到多克隆位点中的lncRNA/基因。
代表性客户文章
- Li Z K, Gao L F, Zhu X A, et al. LncRNA HOXA-AS3 Promotes the Progression of Pulmonary Arterial Hypertension through Mediation of miR-675-3p/PDE5A Axis[J]. Biochemical Genetics, 2021: 1-15.
- Zhang T, Liu H, Mao R, et al. The lncRNA RP11-142A22. 4 promotes adipogenesis by sponging miR-587 to modulate Wnt5β expression[J]. Cell death & disease, 2020, 11(6): 1-12.
- Deng Z, Ou H, Ren F, et al. LncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells[J]. Biological Research, 2020, 53(1): 1-11.
- Xu Y, Fang H, Xu Q, et al. LncRNA GAS5 inhibits NLRP3 inflammasome activation-mediated pyroptosis in diabetic cardiomyopathy by targeting miR-34b-3p/AHR[J]. Cell Cycle, 2020, 19(22): 3054-3065.
- Fang G, Wang J, Sun X, et al. LncRNA MAGI2-AS3 is downregulated in the distant recurrence of hepatocellular carcinoma after surgical resection and affects migration and invasion via ROCK2[J]. Annals of hepatology, 2020, 19(5): 535-540.
产品或服务优势:
1、非病毒载体,有NeoR或EGFP等不同标记的骨架可选
2、可根据客户的不同需求构建不同的表达载体,能够保证所克隆序列的正确性及完整性
3、可在哺乳动物细胞内高效过表达
■ 定制构建载体,请点击“委托技术服务”,提交您的定制需求;
■订购空载体,需点击“委托技术服务”,提交您的需求。
技术支持或应用实例
A、qRT-PCR验证结果图展示
以GAPDH作为内参,选用293T、A549、Hela细胞作为过表达对象,构建好的lncRNA1、2、3过表达载体分别在这3种细胞内过表达,qRT-PCR验证结果如图所示:
图1-3 LncRNA1、2、3过表达载体分别在293T、A549和Hela细胞内过表达的qRT-PCR验证结果图
B、qRT-PCR数据展示
表1
表2
cell | vector | CqGAPDH | CqLnc2 | relative expression |
---|---|---|---|---|
293T | Blank vector | 18.62 | 33.70 | 1.00 |
293T | lnc2 vector | 18.25 | 17.33 | 65536.00 |
A549 | Blank vector | 15.99 | 32.47 | 1.00 |
A549 | lnc2 vector | 16.44 | 15.14 | 225327.94 |
Hela | Blank vector | 16.72 | 33.10 | 1.00 |
Hela | lnc2 vector | 16.79 | 17.90 | 39284.34 |
表3
cell | vector | CqGAPDH | CqLnc3 | relative expression |
---|---|---|---|---|
293T | Blank vector | 17.51 | 25.87 | 1.00 |
293T | lnc3 vector | 17.53 | 12.56 | 10321.27 |
A549 | Blank vector | 17.99 | 33.83 | 1.00 |
A549 | lnc3 vector | 18.36 | 9.71 | 23508296.55 |
Hela | Blank vector | 16.99 | 25.85 | 1.00 |
Hela | lnc3 vector | 17.81 | 10.29 | 85087.92 |