
short hairpin RNA(shRNA)是化学合成siRNA之外的另一种RNAi实验方法常用工具。锐博生物shRNA载体骨架为pRNAT-U6.1/Neo,转染细胞后, shRNA在U6启动子的调控下表达,并最终剪切形成siRNA duplex起作用。载体上另外GFP标签,可用于检测确认质粒的转染情况。
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