micrON™ miRNA agomir是经过特殊化学修饰的miRNA激动剂，通过模拟内源性miRNA 进入miRISC复合物来调节靶基因mRNA 的表达而发挥作用。与普通的miRNA mimic相比，miRNA agomir在动物体内具有更高的稳定性和miRNA活性，更易通过细胞膜、组织间隙而富集于靶细胞，它可以用系统全身或局部注射等方法进行给药。锐博生物提供最新miRBase 数据库中人、小鼠、大鼠的所有miRNA agomir，并不断补充更新，其它物种的miRNA agomir及miRBase未收录的成熟体miRNA agomir需定制，最小订购量为10 nmol。
产品优势
1、低毒性，全球领先的自主专利技术
2、经化学修饰，可直接注射于实验动物，无需转染试剂
3、相比于病毒载体，合成周期短，便于后续转化医学中核酸药开发
4、更稳定，更易通过细胞膜、组织间隙
技术支持
皮下移植SMMC-7721 细胞到NOD-SCID 小鼠，2 周后尾微静脉注射胆固醇修饰及Cy3 荧光标记的miR-199a agomir （使用脂质给药试剂），每3 天一次，共7 次。三周后进行microPET/CT 成像，agomiR-199a 处理后肝肿瘤吸收的[18F]FDG（氟代脱氧葡萄糖）减少，即肿瘤生长减缓。Zhang L, et al. EMBO J. 2015.
b. 以5u mol/kg 一天的剂量给一只小鼠静脉注射miR-125a agomir，21 天后小鼠脊髓进行HE 染色和Luxol Fast Blue 染色，结果显示miR-125a agomir 改善CNS 炎症和髓鞘脱失情况。Pan W, et al. Nat commun, 2015.
对M-KO 小鼠的右后肢腓肠肌进行肌肉注射0.02ml 20uM miR-329 agomir，左后肢注射CTR agomir（对照组），4 天后收集肌肉样本，结果显示miR-329 组DLK1 蛋白显著减少。Gao Y, et al. PNAS, 2015.
代表性客户文章
- Zheng K, Hu F, Zhou Y, et al. miR-135a-5p mediates memory and synaptic impairments via the Rock2/Adducin1 signaling pathway in a mouse model of Alzheimer’s disease[J]. Nature Communications, 2021, 12(1): 1-16.
- Sun Y, Wang Q, Zhang Y, et al. Multigenerational maternal obesity increases the incidence of HCC in offspring via miR-27a-3p[J]. Journal of Hepatology, 2020, 73(3): 603-615.
- Chen J, Liu A, Wang Z, et al. LINC00173. v1 promotes angiogenesis and progression of lung squamous cell carcinoma by sponging miR-511-5p to regulate VEGFA expression[J]. Molecular cancer, 2020, 19: 1-19.
- Zhang S B, Lin S Y, Liu M, et al. CircAnks1a in the spinal cord regulates hypersensitivity in a rodent model of neuropathic pain[J]. Nature communications, 2019, 10(1): 1-16.
- Hou Z, Qin X, Hu Y, et al. Longterm exercise-derived exosomal miR-342-5p: a novel exerkine for cardioprotection[J]. Circulation research, 2019, 124(9): 1386-1400.
- Wang C, Xu W, An J, et al. Poly (ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2[J]. Nature communications, 2019, 10(1): 1-12.
- Cui C, Wang Y, Liu J, et al. A fungal pathogen deploys a small silencing RNA that attenuates mosquito immunity and facilitates infection[J]. Nature communications, 2019, 10(1): 1-10.
订购产品
| 产品编号 | 产品名称 | 物种 | 目录价 | 数量 | 操作 |
|---|---|---|---|---|---|
| miR40000830-4-5 | micrON rno-miR-125b-5p agomir,in vivo,5nmol | rat | ¥2,400.00 | ||
| miR40000537-4-5 | micrON mmu-miR-27a-3p agomir,in vivo,5nmol | mouse | ¥2,400.00 | ||
| miR40004715-4-5 | micrON rno-miR-27a-5p agomir,in vivo,5nmol | rat | ¥2,400.00 | ||
| miR40004324-4-5 | micrON mmu-miR-181d-5p agomir,in vivo,5nmol | mouse | ¥2,400.00 | ||
| miR40000140-4-5 | micrON mmu-miR-128-3p agomir,in vivo,5nmol | mouse | ¥2,400.00 | ||
| miR40000157-4-5 | micrON mmu-miR-145a-5p agomir,in vivo,5nmol | mouse | ¥2,400.00 | ||
| miR40000243-4-5 | micrON hsa-miR-148a-3p agomir,in vivo,5nmol | human | ¥2,400.00 | ||
| miR40000246-4-5 | micrON mmu-miR-122-5p agomir,in vivo,5nmol | mouse | ¥2,400.00 | ||
| miR40001081-4-10 | micrON mmu-miR-196b-5p agomir,in vivo,10nmol | mouse | ¥3,600.00 | ||
| miR40003326-4-5 | micrON hsa-miR-663a agomir,in vivo,5nmol | human | ¥2,400.00 |
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