micrOFF™ miRNA antagomir是经过特殊化学修饰的miRNA拮抗剂，通过与体内的成熟miRNA强竞争性结合，阻止miRNA 与其靶基因mRNA的互补配对，抑制miRNA发挥作用。miRNA antagomir均为miRNA 成熟链的反向互补序列，全链进行甲基化修饰，在5’端和3’端分别有2个和4个碱基硫代修饰，并在3’端连接有高亲和性胆固醇修饰。与普通抑制剂相比，micrOFF™ miRNA antagomir在动物实验和细胞实验中具有更高的稳定性和抑制效果，更易通过细胞膜、组织间隙而富集于靶细胞，在动物实验中可使用生理盐水等溶解后直接注射以进行系统性给药或局部给药。锐博生物提供最新miRBase 数据库中人、小鼠、大鼠的所有miRNA antagomir，并不断补充更新，其它物种的miRNA antagomir及miRBase未收录的成熟体miRNA antagomir需定制，最小订购量为10 nmol。
产品优势
1、低毒性，全球领先的自主专利技术
2、经化学修饰，可直接注射于实验动物，无需转染试剂
3、相比于病毒载体，合成周期短，便于后续转化医学中核酸药开发
4、更稳定，更易通过细胞膜、组织间隙
技术支持
miRNA-328 antagomir 按照80mg/kg 的给药量连续给药3 天通过尾静脉注射到WT 及其TG 小鼠,4 周以后检测。miRNA-328 的升高可以诱发心律失常，miRNA-328 调控钙通道的CACNA1C 蛋白。 Lu Y, et al. Circulation, 2010.
miR-151 的高表达显著增加肝癌细胞的迁移，侵袭与转移，通过利用antagomir 抑制miRNA 的高表达，从而使肿瘤细胞转移得到抑制。 Ding J, et al. Nat Cell Biol, 2010.
对蝗虫注射antagomir-276 后，孵化率、胚胎期分布等均发生变化。 He J, et al. Proc Natl Acad Sci USA. 2016.
代表性客户文章
- Jiang F, Chen Q, Wang W, et al. Hepatocyte-derived extracellular vesicles promote endothelial inflammation and atherogenesis via microRNA-1[J]. Journal of hepatology, 2020, 72(1): 156-166.
- Zhao Y, Zhao M F, Jiang S, et al. Liver governs adipose remodelling via extracellular vesicles in response to lipid overload[J]. Nature communications, 2020, 11(1): 1-17.
- Chen J, Liu A, Wang Z, et al. LINC00173. v1 promotes angiogenesis and progression of lung squamous cell carcinoma by sponging miR-511-5p to regulate VEGFA expression[J]. Molecular cancer, 2020, 19: 1-19.
- Zhang S B, Lin S Y, Liu M, et al. CircAnks1a in the spinal cord regulates hypersensitivity in a rodent model of neuropathic pain[J]. Nature communications, 2019, 10(1): 1-16.
- Cheng M, Yang J, Zhao X, et al. Circulating myocardial microRNAs from infarcted hearts are carried in exosomes and mobilise bone marrow progenitor cells[J]. Nature communications, 2019, 10(1): 1-9.
- Li X, Fu Z, Liang H, et al. H5N1 influenza virus-specific miRNA-like small RNA increases cytokine production and mouse mortality via targeting poly (rC)-binding protein 2[J]. Cell research, 2018, 28(2): 157-171.
- Liu F, Chen J, Wang P, et al. MicroRNA-27a controls the intracellular survival of Mycobacterium tuberculosis by regulating calcium-associated autophagy[J]. Nature communications, 2018, 9(1): 1-14.
订购产品
| 产品编号 | 产品名称 | 物种 | 目录价 | 数量 | 操作 |
|---|---|---|---|---|---|
| miR30000527-4-5 | micrOFF mmu-miR-16-5p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30000830-4-5 | micrOFF rno-miR-125b-5p antagomir,in vivo,5nmol | rat | ¥1,200.00 | ||
| miR30000530-4-5 | micrOFF mmu-miR-21a-5p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30000537-4-5 | micrOFF mmu-miR-27a-3p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30000123-4-5 | micrOFF mmu-miR-1a-3p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30000584-4-5 | micrOFF mmu-miR-339-5p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30001081-4-5 | micrOFF mmu-miR-196b-5p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30000159-4-5 | micrOFF mmu-miR-149-5p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30000677-4-5 | micrOFF mmu-miR-7a-5p antagomir,in vivo,5nmol | mouse | ¥1,200.00 | ||
| miR30001081-4-10 | micrOFF mmu-miR-196b-5p antagomir,in vivo,10nmol | mouse | ¥1,800.00 |
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