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micrOFF™ miRNA inhibitor是化学合成经特殊修饰的miRNA 抑制剂,通过特异性结合成熟miRNA 分子而抑制其作用,调低细胞中miRNA所致的调控作用。miRNA 功能缺失性(loss-of-function)研究常用来筛选miRNA 的靶位点,筛选调控某一基因表达的miRNA,筛选影响细胞生长、增殖过程的miRNA等。

miRNA inhibitor仅需用转染试剂包裹即可转染进细胞,无需构建载体的繁琐操作,无需病毒防护方面的担忧,通过转染对照即可观察转染效率。锐博生物提供miRBase 数据库中人、小鼠、大鼠的所有miRNA inhibitor,并不断补充更新,其他物种的miRNA inhibitor 需定制,最小订购量为10nmol,含有末端修饰的inhibitors 最小起订量为10nmol。

如何选择miRNA对照:NC对照名称中的#19、#22、#24代表序列碱基长度,建议选择与目的基因长度最相近的一种,若为大鼠细胞则建议选#24。

产品优势

1、方便高效,瞬转进入细胞内即可发挥作用
2、操作简单,只需用转染试剂包裹即可转染进入细胞
3、实验周期短
4、应用广泛

技术支持
以miRNA-192 为例,A549 细胞分别用miRNA-192 的mimic 和inhibitor 处理48h 后检测细胞增殖,结果发现miRNA-192 mimic (miR-192 组) 能够促进细胞增殖。Feng S, et al. Nucleic Acids Res, 2011.


SH-SY5Y 转染miR-1285 inhibitor 后,通过qPCR 方法检测到p53 基因的mRNA 表达水平显著升高(A),而蛋白水平亦有相应影响(B)。Tian S, et al. Biochem Biophys Res Commun, 2010.

代表性客户文章
  • Xing S, Tian Z, Zheng W, et al. Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1[J]. Molecular Cancer, 2021, 20(1): 1-19.
  • Yang Z, Wang W, Zhao L, et al. Plasma cells shape the mesenchymal identity of ovarian cancers through transfer of exosome-derived microRNAs[J]. Science advances, 2021, 7(9): eabb0737.
  • Zhang X, Yuan S, Li H, et al. The double face of miR-320: cardiomyocytes-derived miR-320 deteriorated while fibroblasts-derived miR-320 protected against heart failure induced by transverse aortic constriction[J]. Signal transduction and targeted therapy, 2021, 6(1): 1-12.
  • Zheng K, Hu F, Zhou Y, et al. miR-135a-5p mediates memory and synaptic impairments via the Rock2/Adducin1 signaling pathway in a mouse model of Alzheimer’s disease[J]. Nature Communications, 2021, 12(1): 1-16.
  • Yu L, Sui B, Fan W, et al. Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1‐targeting miRNA‐92a‐1‐5p[J]. Journal of extracellular vesicles, 2021, 10(3): e12056.
  • Xie Y, Zhang X, Xu P, et al. Aberrant oligodendroglial LDL receptor orchestrates demyelination in chronic cerebral ischemia[J]. The Journal of clinical investigation, 2021, 131(1).
  • Zheng Z, Zhang X, Huang B, et al. Site-1 protease controls osteoclastogenesis by mediating LC3 transcription[J]. Cell Death & Differentiation, 2021: 1-18.

订购产品

miRNA inhibitors
产品编号 产品名称物种目录价数量操作
miR20000435-1-5 micrOFF hsa-miR-143-3p inhibitor, 5nmolhuman¥500.00
miR20000437-1-5 micrOFF hsa-miR-145-5p inhibitor, 5nmolhuman¥500.00
miR20000449-1-5 micrOFF hsa-miR-146a-5p inhibitor, 5nmolhuman¥500.00
miR20000455-1-5 micrOFF hsa-miR-185-5p inhibitor, 5nmolhuman¥500.00
miR20000076-1-5 micrOFF hsa-miR-21-5p inhibitor, 5nmolhuman¥500.00
miR2190509013442-1-5 micrOFF hsa-miR-7-5p inhibitor (新序列),5nmolhuman¥500.00
miR20000092-1-5 micrOFF hsa-miR-92a-3p inhibitor, 5nmolhuman¥500.00
miR20000093-1-5 micrOFF hsa-miR-93-5p inhibitor, 5nmolhuman¥500.00
miR20000617-1-5 micrOFF hsa-miR-200c-3p inhibitor, 5nmolhuman¥500.00
miR20000667-1-5 micrOFF mmu-miR-33-5p inhibitor, 5nmolmouse¥500.00
miRNA inhibitors对照
产品编号 产品名称目录价数量操作
miR02103S-1-5 micrOFF inhibitor NC #22 (5Cy5 ),5nmol¥780.00
miR0190520020126 micrOFF inhibitor NC #22(5Cy3), in vivo, 5nmol¥936.00
miR2N0000001-1-10 micrOFF inhibitor NC #22, 10nmol¥680.00
miR2N0000002-1-10 micrOFF inhibitor NC #24, 10nmol¥680.00
miR2N0000001-1-5 micrOFF inhibitor NC #22, 5nmol¥400.00
miR2N0000002-1-5 micrOFF inhibitor NC #24, 5nmol¥400.00
miR2N0000003-1-5 micrOFF inhibitor NC #19, 5nmol¥400.00
miR02102-1-1 micrOFF inhibitor NC #22 (5Cy3 ),1nmol¥240.00
miR02104-1-1 micrOFF inhibitor NC #22 (5FAM ),1nmol¥240.00
miR02202-1-1 micrOFF inhibitor NC #24 (5Cy3 ),1nmol¥240.00
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