micrOFF™ miRNA inhibitor是化学合成经特殊修饰的miRNA 抑制剂，通过特异性结合成熟miRNA 分子而抑制其作用，调低细胞中miRNA所致的调控作用。miRNA 功能缺失性（loss-of-function）研究常用来筛选miRNA 的靶位点，筛选调控某一基因表达的miRNA，筛选影响细胞生长、增殖过程的miRNA等。锐博生物提供最新miRBase 数据库中人、小鼠、大鼠的所有miRNA inhibitor，并不断补充更新，其它物种的miRNA inhibitor及miRBase未收录的成熟体miRNA inhibitor需定制，最小订购量为10 nmol。
miRNA inhibitor仅需用转染试剂包裹即可转染进细胞，无需构建载体的繁琐操作，无需病毒防护方面的担忧，通过转染对照即可观察转染效率。
如何选择miRNA对照：锐博生物所有预制inhibitor NC均与人、小鼠、大鼠基因组无同源性（其它物种可联系我们分析确认与目标实验物种无同源性的NC产品），刚开始相关实验时，建议在预实验中比较不同的NC（如#22、#24、#19等）对检测指标的影响并选择没有影响或影响较小的NC进行正式实验。
产品优势
1、方便高效，瞬转进入细胞内即可发挥作用
2、操作简单，只需用转染试剂包裹即可转染进入细胞
3、实验周期短
4、应用广泛
技术支持
以miRNA-192 为例，A549 细胞分别用miRNA-192 的mimic 和inhibitor 处理48h 后检测细胞增殖，结果发现miRNA-192 mimic (miR-192 组) 能够促进细胞增殖。Feng S, et al. Nucleic Acids Res, 2011.
SH-SY5Y 转染miR-1285 inhibitor 后，通过qPCR 方法检测到p53 基因的mRNA 表达水平显著升高(A)，而蛋白水平亦有相应影响(B)。Tian S, et al. Biochem Biophys Res Commun, 2010.
代表性客户文章
- Xing S, Tian Z, Zheng W, et al. Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1[J]. Molecular Cancer, 2021, 20(1): 1-19.
- Yang Z, Wang W, Zhao L, et al. Plasma cells shape the mesenchymal identity of ovarian cancers through transfer of exosome-derived microRNAs[J]. Science advances, 2021, 7(9): eabb0737.
- Zhang X, Yuan S, Li H, et al. The double face of miR-320: cardiomyocytes-derived miR-320 deteriorated while fibroblasts-derived miR-320 protected against heart failure induced by transverse aortic constriction[J]. Signal transduction and targeted therapy, 2021, 6(1): 1-12.
- Zheng K, Hu F, Zhou Y, et al. miR-135a-5p mediates memory and synaptic impairments via the Rock2/Adducin1 signaling pathway in a mouse model of Alzheimer’s disease[J]. Nature Communications, 2021, 12(1): 1-16.
- Yu L, Sui B, Fan W, et al. Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1‐targeting miRNA‐92a‐1‐5p[J]. Journal of extracellular vesicles, 2021, 10(3): e12056.
- Xie Y, Zhang X, Xu P, et al. Aberrant oligodendroglial LDL receptor orchestrates demyelination in chronic cerebral ischemia[J]. The Journal of clinical investigation, 2021, 131(1).
- Zheng Z, Zhang X, Huang B, et al. Site-1 protease controls osteoclastogenesis by mediating LC3 transcription[J]. Cell Death & Differentiation, 2021: 1-18.
订购产品
miRNA inhibitors
| 产品编号 | 产品名称 | 物种 | 目录价 | 数量 | 操作 |
|---|---|---|---|---|---|
| miR20000435-1-5 | micrOFF hsa-miR-143-3p inhibitor, 5nmol | human | ¥500.00 | ||
| miR20000437-1-5 | micrOFF hsa-miR-145-5p inhibitor, 5nmol | human | ¥500.00 | ||
| miR20000449-1-5 | micrOFF hsa-miR-146a-5p inhibitor, 5nmol | human | ¥500.00 | ||
| miR20000455-1-5 | micrOFF hsa-miR-185-5p inhibitor, 5nmol | human | ¥500.00 | ||
| miR20000076-1-5 | micrOFF hsa-miR-21-5p inhibitor, 5nmol | human | ¥500.00 | ||
| miR2190509013442-1-5 | micrOFF hsa-miR-7-5p inhibitor，5nmol | human | ¥500.00 | ||
| miR20000092-1-5 | micrOFF hsa-miR-92a-3p inhibitor, 5nmol | human | ¥500.00 | ||
| miR20000093-1-5 | micrOFF hsa-miR-93-5p inhibitor, 5nmol | human | ¥500.00 | ||
| miR20000617-1-5 | micrOFF hsa-miR-200c-3p inhibitor, 5nmol | human | ¥500.00 | ||
| miR20000667-1-5 | micrOFF mmu-miR-33-5p inhibitor, 5nmol | mouse | ¥500.00 |
miRNA inhibitors对照
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